ADRENOCORTICOTROPIC HORMONE
adrenocorticotropic hormone
CAS: 9002-60-2
Molecular Formula: C207H308N56O58S
ADRENOCORTICOTROPIC HORMONE - Names and Identifiers
ADRENOCORTICOTROPIC HORMONE - Physico-chemical Properties
| Molecular Formula | C207H308N56O58S |
| Molar Mass | 4541.1 |
| Solubility | H2O: 1mg/mL, clear, colorless |
| Appearance | powder |
| Merck | 13,136 |
| Storage Condition | −20°C |
| Physical and Chemical Properties | This product is from the pig, cattle, sheep and other anterior pituitary gland extract a polypeptide drugs, white or light yellow powder, odorless, hygroscopic, soluble in water, soluble in 70% of acetone and 70% of ethanol, dry and weak acidic solution is more stable, strong acid or alkaline in easy inactivation. 100 C heating stability. This product is composed of 39 amino acid residues, species difference is only in the 25-33, 1-24 fragment has all the activity, the 24th amino acid after the part only maintains the stability of the entire polypeptide structure. There is a high degree of α-helical structure in solution. Partial hydrolysis by pepsin is still active. This product is invalid by oral administration; The effect of intramuscular injection is poor; The effect of intravenous injection is fast, but the half-life is only 15min, and the maintenance time is 1-3H. |
ADRENOCORTICOTROPIC HORMONE - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. R40 - Limited evidence of a carcinogenic effect |
| Safety Description | S22 - Do not breathe dust. S36 - Wear suitable protective clothing. |
| WGK Germany | 3 |
| RTECS | GM7900000 |
| FLUKA BRAND F CODES | 8-10 |
| HS Code | 2937190000 |
ADRENOCORTICOTROPIC HORMONE - Reference
| Reference Show more | 1. Zhao Hui, Lin Lin, Zhu Yangchun, etc. Effect of Bushen Tongdu capsule on hypothalamus-pituitary-adrenal axis in rats with collagen-induced arthritis [J]. Journal of Hubei University of Traditional Chinese Medicine, 2015, 17(1):13-15. 2. [IF = 3.575] Xiaoyan Ma et al."Rapid and sustained restoration of ASTM functions by ketamine in depression model mice. BIOCHEMICAL AND Biomedical RESEARCH COMMUNICATIONS. 2022 Apr;: |
ADRENOCORTICOTROPIC HORMONE - Reference Information
| overview | adrenocorticotropic hormone (adrenocorticotropichormone,adrenocorticotropin,ACTH) is a polypeptide hormone extracted from the anterior lobe of mammalian pituitary gland. This product can also be manufactured by synthetic method. Corticotropin is a white or light yellow lyophilized preparation. Easily soluble in water and ethanol. It is more stable in weak acids, and is easily inactivated in strong acids or bases. Porcine corticotropin is composed of 39 amino acids, and its structural formula is shown above. The structure of corticotropin in cattle, sheep, pigs and humans is similar. The 3 1st position of human corticotropin is "serine", and the pig is "leucine", and the 25th position is "aspartic acid" and 33 "Glutamic acid" sometimes becomes "asparagine" and "glutamine". |
| use | hormone drugs. It is used for the diagnosis of adrenaline cortex function, long-term use of corticosteroids to prevent cortical atrophy after stopping the drug, and can also be used to treat certain collagen diseases, severe bronchial asthma, small seizures and myasthenia gravis. Adverse reactions and contraindications: A large number of applications can cause adverse reactions such as hypertension, menstrual disorders, headache, diabetes, and mental disorders. It can also cause severe allergic reactions. Tuberculosis, hypertension, diabetes, vascular sclerosis, ulcer disease and pregnant women are prohibited. Intravenous drip is not compatible with alkaline injection, otherwise turbidity is easy to occur. Hormonal drugs, promote the secretion of adrenal cortex hormones, used for active rheumatism, rheumatoid arthritis, lupus erythematosus and other collagen diseases. Clinical use is similar to cortisone. |
| production method | a hormone extracted from the anterior pituitary lobe of livestock pigs, cattle or sheep, belonging to peptides. methods preparation of adenohypophysis dry powder by 1. acid acetone extraction method fresh pig pituitary gland was quickly put into acetone, soaked and dehydrated for three times for 24 hours each time until the pituitary gland was pink and hardened. The adenohypophysis and neurohypophysis were strictly separated and stored in acetone respectively. Otherwise, when the adenohypophysis contains the neurohypophysis, the vasopressin will exceed the limit. During preparation, the adenohypophysis is taken out, dried, ground, and screened through 40-60 mesh to obtain adenohypophysis dry powder (water content should be less than 6%). Porcine pituitary gland [acetone] → adenohypophysis [drying and grinding] → extraction of adenohypophysis dry powder corticotropin 10kg of adenohypophysis powder is added with acetone to make paste, then the total amount of acetone is 84L, 36L of 1mol/L hydrochloric acid is added under stirring, maintained at 50 ℃ for 30min, rapidly lowered the temperature to below 30 ℃ for filtration, and the filtrate is cooled to about 4 ℃. The filter residue was extracted with 28L acetone and 12L 0.1 mol/L hydrochloric acid again and filtered. Merge the filtrate twice, and when the temperature is about 4 ℃, filter repeatedly until clarification. Adenohypophysis dry powder [projiong, HCl]→[50 ℃, 30min] extraction [filtration] →[4 ℃] preparation of filtrate corticotropin finished product by volume adding 0.3% 5 mol/L sodium chloride solution, stirring evenly, adding 8-10 times the amount of acetone, stirring for 10min and then placing for 5min. Remove the acetone clear liquid, filter the precipitate with No. 3 vertical melting funnel, and then wash with acetone and anhydrous ether twice respectively, and dry in vacuum to obtain the finished product of corticotropin. Filtrate [NaCl, Acetone] → [Vacuum Drying] Corticotropin Finished Method 2. Extraction of Adenohypophysis by Cross-linked Carboxymethyl Cellulose (CMC) Refining Method 1kg of adenohypophysis dry powder is taken, 20L of 0.5mol/L acetic acid (preheated to 50 ℃ first) is added, stirred, slowly added about 100ml of 50% sulfuric acid dropwise, adjusted the pH2-2.4, heated to 70-75 ℃, maintained for 10min, and quickly cooled to below 20 ℃, add about 400-600g of diatomite soaked overnight with 0.5mol/L acetic acid, filter and refrigerate the filtrate. The filter residue was treated again by the same method (0.5mol/L acetic acid 15L, 50% sulfuric acid 20ml, diatomite 200g). The residue was washed with 0.5mol/L acetic acid 2L and filtered. Merge the filtrate for 3 times, adjust the pH to 3.1-3.4 with 10% sodium hydroxide solution, filter to clarify with Brinell funnel and No. 4 vertical melting funnel respectively, and refrigerate the filtrate overnight. Adenohypophysis dry powder [HAc, H2SO4]→[pH2-2.4, 70-75 ℃] Adenohypophysis extract [NaOH]→[pH3.1-3.4] filtrate adsorption and elution After adjusting the pH of the refrigerated filtrate to 3.1, 40ml of polysorbate 80 and 200g of cross-linked CMC are added, adsorbed under stirring at 3 ℃ for 12h, and refrigerated overnight. Then the supernatant is sucked off, the precipitate is filtered with No.3 vertical melting funnel, CMC is collected, CMC is washed with 0.1mol/L acetic acid 10L and distilled water 10L respectively, CMC is drained at about 15 ℃, and then 800ml 0.15mol/L hydrochloric acid is eluted once. Merge the filtrate, add about 2000g of 717 or Zeroli FF anion exchange resin, immediately adjust the pH to above 3, filter, wash the resin with 1L of 0.1mol/L acetic acid, merge the filtrate, adjust the pH to 3.1 with 0.5 mol/L hydrochloric acid or sodium hydroxide, and refrigerate overnight. Adenohypophysis filtrate [CMC, pH3.1]→[3 ℃,12h] adsorbate [HAc, distilled water, HCl]→[pH3.1,15 ℃] eluent secondary adsorption and elution Add 10-12ml polysorbate 80 to refrigerated eluent, stir well, add 30g CMC, stir and adsorb at 1-5 ℃ for 12h, refrigerate overnight. The next day, 2/3 of the supernatant was sucked off, 1/3 was left to be filtered with No. 4 vertical melting funnel, CMC was collected, 0.1mol/L acetic acid 4L and distilled water 4L were used respectively, washed twice each, drained, 1.3L of 0.01mol/L ammonium acetate solution with pH4.8 was added, stirred below 15 ℃ for 15min, filtered and drained, and washed once with distilled water 1.2L, add 1.3L of 0.1mol/L ammonium acetate solution with pH6.7, stir at 10-15 ℃ for 15min, filter, wash twice with 3L distilled water, and drain. 0.15mol/L hydrochloric acid 1L was stirred and eluted at 25 ℃ for 1.5h, filtered, CMC was washed once with 160ml of 0.15mol/L hydrochloric acid, and the lotion was incorporated into the eluent. Eluate [CMC]→[1-5 ℃,12h] adsorbate [HAc, distilled water, ammonium acetate] →[10-15 ℃] adsorbate [HCl, 25 ℃]→ purification of eluate corticotropin with human 717 or Zerolit FF anion exchange resin of about 300g, adjust pH to about 4, and filter. Wash the resin once with 0.1mol/L acetic acid 150ml, combine the washing liquid with the filtrate, add about 84g of 732 cation exchange resin, adjust the pH back to about 3, filter, wash the resin once with 0.1mol/L acetic acid 50ml, merge the washing liquid into the filtrate, freeze-dry, and obtain refined corticotropin. Eluate [anion and cation exchange resin] → [lyophilized] refined corticotropin. |
| category | toxic substances |
| flammability hazard characteristics | flammability |
| storage and transportation characteristics | warehouse ventilation and low temperature drying; separate from food raw materials |
| fire extinguishing agent | dry powder, foam, sand, carbon dioxide, mist water |
| toxic substance data | information provided by: pubchem.ncbi.nlm.nih.gov (external link) |
Last Update：2024-04-09 20:52:54
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Mobile: 18021002903
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